Journal: iScience

Article Title: TP53 upregulation via aurora kinase inhibition overcomes primary failure to venetoclax in BCL2- rearranged lymphomas

doi: 10.1016/j.isci.2025.112584

Figure Lengend Snippet: AURK-A, AURK-B, or MDM2 inhibitor in combination with Venetoclax induces synergism in BCL2 -rearranged lymphoma cells (A) Cells were treated for 48 h with MLN8237 (MLN, 50 nM), AZD2811 (AZD, 40 nM), LY3295668 (LY, 250 nM), and/or venetoclax (VEN, 40 nM) in WSU-NHL ( BCL2+/MYC-/BCL6- ), DoHH2 ( BCL2+/MYC+/BCL6- ), and VAL ( BCL2+/MYC+/BCL6+ ) cells. Raji cells ( BCL2-/MYC+ ) were shown in comparison. Statistical significance was determined by multiple unpaired t-test. Data are represented as mean ± SD. (B) Focused cytotoxicity assays of MLN8237, venetoclax, or combination treatment in resistant BCL2 -rearranged cells WSU-NHL, DoHH2, and VAL. BCL2 -wild-type Raji cells were shown in comparison. ATP levels were quantified and normalized to vehicle control, and each data point is mean of triplicate wells and graphs are representative of three independent experiments. Data are represented as mean ± SD. (C) Synergism analysis using Combenefit in DoHH2 and VAL cells. (D) Focused cytotoxicity assays of venetoclax, RG-7388, or combination treatment of VEN+RG-7388 in VAL and DoHH2. Cells were treated for 48 h with RG-7388 (50 nM), venetoclax (40 nM), or combination (50 nM RG-7388/40 nM VEN) in VAL and DoHH2 cells. Values are normalized and an average of three independent experiments. Statistical significance was determined by one-way ANOVA with multiple comparisons. Data are represented as mean ± SD. (∗∗∗∗ p < 0.0001, ∗∗∗ p < 0.001, ∗∗ p < 0.01, and ∗ p < 0.05). See also and .

Article Snippet: The Burkitt lymphoma cell lines Raji (CLS Cat# 300359/p25072_RAJI, RRID:CVCL_0511) and Ramos (CLS Cat# 302007/p32087_Ramos, RRID:CVCL_0597) were purchased from ATCC (Manassas, VA, USA).

Techniques: Comparison, Control

Journal: PLoS ONE

Article Title: A Novel BAT3 Sequence Generated by Alternative RNA Splicing of Exon 11B Displays Cell Type-Specific Expression and Impacts on Subcellular Localization

doi: 10.1371/journal.pone.0035972

Figure Lengend Snippet: HeLa cells (Adenocarcinoma), the human melanoma cell line MelJuSo, the B lymphoma Raji and monocyte-derived dendritic cells (moDCs) were plated on coverslips and stained for BAT3 using a polyclonal serum against a C-terminal peptide (middle lane). Cell nuclei (left lane) were visualized with DAPI (A) or 7AAD (B). Merged images are shown in the right lane. A. Immunofluorescence staining was evaluated with a standard fluorescence microscope and B. by confocal microscopy. Scale bars = 10 µm. C. Nuclear and cytosolic staining of endogenous BAT3 in Raji cells was evaluated in 10 single cells using ImageJ. MFI, mean fluorescence intensity per region of interest D. Western blot analysis of subcellular fractions from Raji and HeLa cells. Nuclei (N) and cytoplasm (C) were separated by SDS-PAGE and immunoblotted for BAT3, GADPH (cytosolic marker) and histone H3 (nuclear marker).

Article Snippet: The B lymphoma cell line Raji was cultured in RPMI and dendritic cells were grown in VLE-RPMI supplemented with antibiotics and glutamine (Biochrom AG, Berlin, Germany).

Techniques: Derivative Assay, Staining, Immunofluorescence, Fluorescence, Microscopy, Confocal Microscopy, Western Blot, SDS Page, Marker

Journal: PLoS ONE

Article Title: A Novel BAT3 Sequence Generated by Alternative RNA Splicing of Exon 11B Displays Cell Type-Specific Expression and Impacts on Subcellular Localization

doi: 10.1371/journal.pone.0035972

Figure Lengend Snippet: A. HeLa cells transfected with BAT3 Δ11B,24 were cultured on coverslips in the presence (lower panel) or absence (upper panel) of leptomycin B (LMB) for 2 h. Cells were subsequently stained with V5 mAb and inspected with standard immunofluorescence microscopy (second panel). Left panel shows DAPI stained nuclei, third panel merging of images and right panel displays phase contrast images. Scale bars = 10 µm. B. Raji cells were cultured for 2 h in the presence (lower panel) or absence (upper panel) of LMB and then plated on coverslips. Cells were subsequently stained with the polyclonal anti-BAT3 serum and with ISCR3 mAb (HLA-DR) for evaluation by immunofluorescence microscopy. Left panel shows DAPI staining, second panel staining for BAT3, third panel staining for HLA-DR and images were merged in the right panel. Scale bars = 5 µm.

Article Snippet: The B lymphoma cell line Raji was cultured in RPMI and dendritic cells were grown in VLE-RPMI supplemented with antibiotics and glutamine (Biochrom AG, Berlin, Germany).

Techniques: Transfection, Cell Culture, Staining, Immunofluorescence, Microscopy

Journal: Frontiers in Oncology

Article Title: Tissue Factor-Targeted “O 2 -Evolving” Nanoparticles for Photodynamic Therapy in Malignant Lymphoma

doi: 10.3389/fonc.2020.524712

Figure Lengend Snippet: The antitumor effect and safety evaluation in vivo . Sections (A–E) correspond to the Raji mouse model. (A) PET imaging performed 24 h before and 24 h after PDT treatment. (B) Quantitative analysis of tumor growth inhibition rate by mean radioactivity of 18F-FDG. *p < 0.05, ***p < 0.001. (C) Relative tumor volume variation of mice treated under different conditions. (D) H&E staining and TUNEL staining of tumors at 24 h after PDT treatment. Apoptotic cells were identified by TUNEL assay (brown) (scale bar 100 μm). (E) H&E stained images of major organ slices (scale bar 200 μm). (F) Tumors at 24 h post-treatment and corresponding TUNEL staining of tumor slides in the Jeko-1 mouse model. (scale bar 50 μm).

Article Snippet: Cells were maintained in RPMI-1640 supplemented with 10% FBS; the Jeko-1 and Raji cell lines were purchased from the China Center for Type Culture Collection (Wuhan University, China) and cultured in an RPMI-1640 culture medium containing 10% FBS at 37°C in a humidified atmosphere enclosing 5% CO 2 .

Techniques: In Vivo, Imaging, Inhibition, Radioactivity, Staining, TUNEL Assay

Journal: eLife

Article Title: Caspase-mediated cleavage of IRE1 controls apoptotic cell commitment during endoplasmic reticulum stress

doi: 10.7554/eLife.47084

Figure Lengend Snippet:

Article Snippet: Cell line (human) , Raji , Genentech , RRID: CVCL_0511 , Burkitt’s lymphoma.

Techniques: Knock-Out, Isolation, Clone Assay, CRISPR, Transfection, Construct, FLAG-tag, shRNA, Recombinant, Caspase-Glo Assay, Cell Viability Assay, Flow Cytometry, Fractionation, Cell Culture, Purification, Software

Journal: International Journal of Molecular Sciences

Article Title: Low-Dose Salinomycin Alters Mitochondrial Function and Reprograms Global Metabolism in Burkitt Lymphoma

doi: 10.3390/ijms26115125

Figure Lengend Snippet: Salinomycin (SAL) reduces mitochondrial membrane potential (MMP) in live Raji cells. ( A ) MMP was assessed using the cationic carbocyanine dye JC-1 after 12 h of SAL treatment at concentrations of 0.25 µM, 0.5 µM, and 5 µM. ( B ) MMP was evaluated using tetramethylrhodamine methyl ester (TMRM) following 36 h of SAL treatment at the same concentrations. A positive control was included by treating cells with 50 µM carbonyl cyanide 3-chlorophenylhydrazone (CCCP) for 1 h. Data are expressed as mean ± SD from three experiments ( n = 3), and statistical significance was assessed using one-way ANOVA (*** p < 0.001, **** p < 0.0001).

Article Snippet: The Burkitt lymphoma Raji cell line (RRID:CVCL_0511) was maintained in RPMI 1640 medium (Corning, NY, USA; cat. #10-040-CVR) supplemented with 10% fetal bovine serum (FBS; Fisher Scientific, Waltham, MA, USA; cat. #SH3007203), 100 U/mL penicillin, and 100 μg/mL streptomycin at 37 °C in a humidified 5% CO 2 atmosphere.

Techniques: Membrane, Positive Control

Journal: International Journal of Molecular Sciences

Article Title: Low-Dose Salinomycin Alters Mitochondrial Function and Reprograms Global Metabolism in Burkitt Lymphoma

doi: 10.3390/ijms26115125

Figure Lengend Snippet: SAL induces the generation of reactive oxygen species (ROS) in Raji cells. ( A ) Mitochondrial superoxide production was detected using MitoSOX dye following 36 h of SAL treatment at concentrations of 0.25 µM, 0.5 µM, and 5 µM. ( B ) Total ROS levels were assessed using CellROX Green reagent after 48 h of SAL treatment at concentrations of 0.25 µM and 0.5 µM. As a positive control, cells were treated with 50 µM CCCP for 1 h. Results are expressed as mean ± SD from three experiments ( n = 3), and statistical significance was evaluated using one-way ANOVA (**** p < 0.0001).

Article Snippet: The Burkitt lymphoma Raji cell line (RRID:CVCL_0511) was maintained in RPMI 1640 medium (Corning, NY, USA; cat. #10-040-CVR) supplemented with 10% fetal bovine serum (FBS; Fisher Scientific, Waltham, MA, USA; cat. #SH3007203), 100 U/mL penicillin, and 100 μg/mL streptomycin at 37 °C in a humidified 5% CO 2 atmosphere.

Techniques: Positive Control

Journal: International Journal of Molecular Sciences

Article Title: Low-Dose Salinomycin Alters Mitochondrial Function and Reprograms Global Metabolism in Burkitt Lymphoma

doi: 10.3390/ijms26115125

Figure Lengend Snippet: SAL suppresses mitochondrial respiration in Raji cells. ( A ) Oxygen consumption rate (OCR) was measured using a Seahorse XFe96 analyzer, following 24 h-long treatment with 0.25 µM, 0.5 µM, or 5 µM SAL. OCR was assessed under basal conditions and after the sequential addition of oligomycin (2.5 µM), carbonyl cyanide-p-trifluoromethoxyphenylhydrazone (FCCP, 1 µM and 1.5 µM), and antimycin A (2.5 µM). The data concerning maximal respiration ( B ), basal respiration ( C ), and ATP production ( D ) have been extracted from the analysis presented in ( A ). Data were normalized to cell number and represent the mean ± SD from experiments ( n = 3). Statistical analysis was performed using one-way ANOVA (** p < 0.01, **** p < 0.0001).

Article Snippet: The Burkitt lymphoma Raji cell line (RRID:CVCL_0511) was maintained in RPMI 1640 medium (Corning, NY, USA; cat. #10-040-CVR) supplemented with 10% fetal bovine serum (FBS; Fisher Scientific, Waltham, MA, USA; cat. #SH3007203), 100 U/mL penicillin, and 100 μg/mL streptomycin at 37 °C in a humidified 5% CO 2 atmosphere.

Techniques:

Journal: International Journal of Molecular Sciences

Article Title: Low-Dose Salinomycin Alters Mitochondrial Function and Reprograms Global Metabolism in Burkitt Lymphoma

doi: 10.3390/ijms26115125

Figure Lengend Snippet: SAL reduces glycolytic activity in Raji cells. ( A ) Extracellular acidification rate (ECAR) was measured using the Seahorse XFe96 analyzer following treatment with 0.25 µM, 0.5 µM, and 5 µM SAL under basal conditions and after sequential injections of oligomycin (2.5 µM), FCCP (1 µM and 1.5 µM), and antimycin A (2.5 µM). ( B , C ) Quantification of basal ECAR ( B ) and glycolytic reserve in response to oligomycin ( C ) was extracted from the ECAR profiles shown in ( A ). Data were normalized to cell number and represent the mean ± SD from three experiments ( n = 3). Statistical analysis was performed using one-way ANOVA (** p < 0.01, ns indicates non-significant differences ( p > 0.05)).

Article Snippet: The Burkitt lymphoma Raji cell line (RRID:CVCL_0511) was maintained in RPMI 1640 medium (Corning, NY, USA; cat. #10-040-CVR) supplemented with 10% fetal bovine serum (FBS; Fisher Scientific, Waltham, MA, USA; cat. #SH3007203), 100 U/mL penicillin, and 100 μg/mL streptomycin at 37 °C in a humidified 5% CO 2 atmosphere.

Techniques: Activity Assay

Journal: International Journal of Molecular Sciences

Article Title: Low-Dose Salinomycin Alters Mitochondrial Function and Reprograms Global Metabolism in Burkitt Lymphoma

doi: 10.3390/ijms26115125

Figure Lengend Snippet: Untargeted metabolomic profiling of Raji cells treated with 0.25 µM SAL, analyzed using SolariX 2xT 7T. ( A ) OPLS-DA (Orthogonal Projections to Latent Structures–Discriminant Analysis) score plot R2X = 0.104, R2Y = 0.878, Q2 = 0.476; CV-ANOVA p = 0.0399. ( B ) OPLS-derived VIP (Variable Importance in Projection) score plot.

Article Snippet: The Burkitt lymphoma Raji cell line (RRID:CVCL_0511) was maintained in RPMI 1640 medium (Corning, NY, USA; cat. #10-040-CVR) supplemented with 10% fetal bovine serum (FBS; Fisher Scientific, Waltham, MA, USA; cat. #SH3007203), 100 U/mL penicillin, and 100 μg/mL streptomycin at 37 °C in a humidified 5% CO 2 atmosphere.

Techniques: Derivative Assay

Journal: International Journal of Molecular Sciences

Article Title: Low-Dose Salinomycin Alters Mitochondrial Function and Reprograms Global Metabolism in Burkitt Lymphoma

doi: 10.3390/ijms26115125

Figure Lengend Snippet: Metabolome of SAL-treated Raji cells. Volcano plots display differentially regulated metabolites, with the most significant ones marked with annotations, based on ( A ) FDR-adjusted p -values ( t -test p = 0.05) and ( B ) unadjusted p -values ( t -test p = 0.05). Blue represents metabolites that are lower in the SAL-treated group, while red denotes metabolites that are higher relative to the vehicle group. Horizontal dashed line indicates p = 0.05.

Article Snippet: The Burkitt lymphoma Raji cell line (RRID:CVCL_0511) was maintained in RPMI 1640 medium (Corning, NY, USA; cat. #10-040-CVR) supplemented with 10% fetal bovine serum (FBS; Fisher Scientific, Waltham, MA, USA; cat. #SH3007203), 100 U/mL penicillin, and 100 μg/mL streptomycin at 37 °C in a humidified 5% CO 2 atmosphere.

Techniques:

Journal: Immunotherapy Advances

Article Title: Characterization of sabatolimab, a novel immunotherapy with immuno-myeloid activity directed against TIM-3 receptor

doi: 10.1093/immadv/ltac019

Figure Lengend Snippet: ADCP activity of sabatolimab in vitro . (A) THP-1 cells (TIM-3 sufficient, control, or TIM-3 deficient, KO) were cultured for 1 hour with Raji TIM-3 o.e. cells and treated with different concentrations of sabatolimab or hIgG4 isotype control in duplicates. ADCP was determined by flow cytometric analysis as % of CFSE + CD11c + cells. Plots depict means ± SD for each condition. (B) THP-1 cells (TIM-3 sufficient, control, or TIM-3 deficient, KO) cultured as in A with with Raji hTIM-3 o.e. cells were treated with sabatolimab or hIgG4 isotype control, with Latrunculin A or Fc-blocking antibody added to some of the wells in duplicates. ADCP was determined by flow cytometric analysis as % of CFSE + CD11c + cells. Plots depict means ± SD for each condition. (C,D) Macrophages differentiated from PBMC-isolated monocytes were cultured for 4 hours with CFSE-labeled leukemia cells (HNT-34, in C or SKM-1, in D) and treated with different concentrations of sabatolimab or hIgG4 isotype control in duplicates. ADCP was determined by flow cytometric analysis as % of CFSE + CD11c + cells. Plots depict means ± SD for each condition. Two separate donors are shown for each cell line. Asterisks mark statistically significant P values between sabatolimab- and isotype control-treated samples ( T test * P < 0.05, ** P < 0.005, *** P < 0.001).

Article Snippet: The Raji human lymphoblast-like cell line, established from a Burkitt’s lymphoma of the left maxilla of an 11-year-old black male [ ], was obtained from Deutsche Sammlung von Mikrooganismen Zellkulturen (DSMZ, Braunschweig, Germany) and engineered to stably express the human TIM-3 at Trenzyme (Konstanz, Germany).

Techniques: Activity Assay, In Vitro, Control, Cell Culture, Blocking Assay, Isolation, Labeling